Journal: bioRxiv
Article Title: Overcoming Immune Checkpoint Inhibitor Resistance via Potent and Selective Dual αvβ6/8 Inhibitors Based on Engineered Lasso Peptides
doi: 10.1101/2025.01.28.635346
Figure Lengend Snippet: Analytical QC check for lassotides purified by preparative HPLC. Purified lassotides were analyzed using a Shimadzu LC-10ADvp HPLC equipped with a Vydac C18 25 cm x 4.6 mm, 5-micron column. Mobile phase was 0.1% TFA in 15-20% CH3CN increased to 35-50% CH3CN over 20 min. A . Lassotide 35 , B . Lassotide 36 , C . Lassotide 38 , D . Lassotide 47 . In all cases, lassotide purity was ≥ 98% in all cases.
Article Snippet: Preparative HPLC was carried out using a Shimadzu LC 8A HPLC equipped with a Vydac 10-15 μm C18, 300 mm x 47 mm, preparative column (0.1-30 g scale), or an Agilent 1100 or 1260 purification system (ChemStation software, Agilent) equipped with an autosampler, multiple wavelength detector, Prep-LC fraction collector and Phenomenex Luna 5 μm C18(2) 150 x 30 mm preparative column (10-100 mg scale).
Techniques: Purification